AAWSAP DIRD, Quantum Computing and Utilizing Organic Molecules in Automation Technology, December 2010

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that the resulting geometry approximates the shape within one DNA turn ( ~ 3.6 nm) in the
x-direction and two helical widths(~ 6 nm, including an inter-helix gap) in they-direction.
To make a molecular design, a scaffold is run exactly once through each helix; performed in
a raster-fill manner, this creates a 'folding path' (Figure 18b). To hold the scaffold in this
shape, helper strands are added to create a regular pattern of antiparallel crossovers (Figure
18c).
Figure 19 illustrates the versatility of shapes-programmed DNA origami with a high yield in
excess of 70%. Each shape seen in Figure 19 uses 7000-base long scaffolds requiring more
than 200 DNA strands for a final molecular weight of 15,000 nucleotides. Thus, the DNA
origami structure has a molecular weight that is l00X that of the original DX model and
nearly 6X larger in geometric construction where 50 billion copies of the pattern are created
at once. With this technique, a device has been created that has a molecular weight of the
component of cells that can synthesize proteins and amino acids- the ribosome. For the first
time, we are now capable of self-assembling structures whose size and complexity rival that
of Nature's most complex self assembled machines.
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Figure 17. (A) Schematic diagram of a 16-column microfluidic DNA synthesizer.
The control lines are shown in red, the fluidic lines in blue, the herringbone
mixers in yellow, and the square profiled binary tree and reactor columns in
green. (8) Close up schematic of the column array (106).
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